Ten Punnett Square Worksheet Ideas for Middle School through AP Levels

Punnett square

The Punnett square worksheet is a great teaching tool for genetics. This worksheet helps students get an idea of the different possible combinations for genetic traits and helps them calculate how likely each combination is. Here are some ideas for using the Punnett square in your classroom.

The Punnett square is a diagram used to make sense out of genetics and inheritance. The purpose of this diagram is to show the different possible combinations of alleles. This is a useful tool you can use to teach biology and probabilities regardless of the level of your students. Here are a few ideas to use the Punnett square in your classroom.

Understanding Dominant And Recessive Alleles

You should talk about genetics and alleles before introducing the Punnett square worksheet in your classroom. Students should ideally also have a good understanding of how to calculate probabilities.

Students should be familiar with genes and understand that genes are a unit of hereditary information while an allele is a possible sequence or variant of a gene.

Punnett square

You should also talk about observable genetic traits, also known as phenotypes. Students should understand that there are dominant and recessive alleles that won’t become phenotypes unless they are combined with another recessive allele. You can introduce the notion of codominant alleles with high school students.

Make sure the Punnett square activities are connected to lessons about genetics, inheritance, and alleles. You can use these activities to introduce these concepts or to help students get a more thorough understanding of genetics and probabilities.

The Punnett Square

The Punnett square is a simple diagram that shows the different possible combinations. Here is an example for the offspring of two organisms with the same Aa allele combination:



Using this worksheet helps students see all the different possibilities and gives them an idea of which phenotype is more likely to occur.

Ten Ideas For Using The Punnett Square Worksheet In Your Classroom

Middle School

Introduce The Punnett Square With Legos

You can use Legos to introduce the Punnett square to your students. This visual approach would be ideal for an activity that you will use to introduce concepts like genetics and alleles.

You need to have Legos with two different shapes to represent the dominant and recessive alleles. Use cups or other small containers to represent the animals or plants that inherit the genetic material.

Start with two cups that contain a different combination of two Lego shapes to represent the parents. Have the students fill out the worksheet with the four possible combinations of Lego shapes.

The students can then place the four different combinations inside of four cups or small containers that represent the offspring.

This approach helps students understand the logic behind the Punnett square and gives them a visual reference you can use once you start talking about alleles.

Plant Genetics

Plant Genetics

Plants are a great example at the middle school level because you can easily identify a phenotype that students will understand, such as the color of a flower. You can even grow flowers in the classroom to illustrate the lesson.

Students can fill out a Punnett square worksheet for plant genetics. The purpose of this activity is to introduce the idea of dominant and recessive alleles and have students get used to seeing a capital letter for the dominant trait and a lowercase letter for the recessive trait.

Create a simple worksheet with four squares and ask students to write the different possible combinations. You can work with different phenotypes:

  • Create a worksheet for a blue flower BB and a blue flower Bb.
  • Create a worksheet for a blue flower Bb and a white flower bb.
  • Create a worksheet for a tall plant TT and a tall plant Tt.
  • Create a worksheet for a tall plant Tt and a small plant tt.

You can then ask students to identify the number of possible combinations and to calculate the probability of a flower being blue or of a plant being tall. You can also have students draw what the plants will look like.

High School

You can introduce advanced ideas at the high school level and connect the Punnett square with more real-life examples. You should introduce concepts like homozygous genes, heterozygous genes, or mutations.

You can also focus on probabilities and have students use a worksheet to calculate the probability of a trait appearing in offspring.

Predicted Outcome And Actual Outcome

Introduce the idea that the predicted outcome of a Punnett square doesn’t always reflect what happens in real life. Students should be aware that these worksheets will show how likely an allele combination is.

Have students use a Punnett square worksheet to predict the outcome of a coin flip or another random event. Once the worksheet is filled out, have students flip a coin and compare the outcome with what the Punnett square predicted.

Guessing The Parents’ Allele Combination

Provide students with different allele combination for the offspring and tell them how frequent each combination is.

Have them use the Punnett square to find the allele combinations of the parents. This is an activity that only takes a few minutes to complete, but it is a great way to brush up on how the Punnett square works and to make sure that students have a solid understanding of inheritance.

Bear Fur Color

You can work with phenotypes that are observable in animals and introduce the idea that there are different possible allele combinations for the same phenotype.

The brown bear fur color is an excellent example since a bear can have BB or Bb alleles and have brown fur. On the other hand, only a bear with a bb allele combination will have black fur.

Have your students use the Punnett square to calculate the probability of offspring having brown or black fur. This problem encourages students to create more complex tables since the bear parents can either have the BB or Bb allele combination.

Eye Color


Predicting eye color is another interesting activity you can introduce at the high school level. Start by making a list of the different possible allele combinations for each eye color.

You can have students calculate the probability of their eye color based on the eye color of their parents, or have students determine the allele combinations of two parents based on the phenotypes of their children.

Eye color is more complex than other phenotypes and gives students an opportunity to create more advanced worksheets that reflect the different possible allele combinations of the parents based on their phenotypes.

You can make things more complicated and combine eye color with hair color.


Have students research different genetic traits and create a presentation on how these traits are inherited. You can have students work in groups and assign a trait to each group.

Students will have to define the trait you assigned to them, explain how it is inherited, and create different Punnett squares that show how the trait can be inherited or skip a generation.

Here are a few examples:

  • A specific hair color.
  • A specific eye color.
  • Tongue rolling.
  • Freckles.
  • Free or attached earlobes.

This project will help students understand how complex genetic inheritance is and will also help them connect what they learned in class with real-world examples.

AP Level

Cat Coat Genetics

Students can look at pictures of cats and predict what the offspring will look like with Punnett squares. This is similar to the bear fur activity, but cat coat genetics are more complex.

Students will have to work with genotypes that affect hair length as well as color. Here are a few facts to help you get started with planning this activity:

  • A cat with short hair will have an LL or Ll genotype.
  • A cat with long hair will have an ll genotype.
  • A cat that is entirely white can have a WW or Ww genotype. A cat with white fur and some colored hair has a ww genotype.
  • White cats can have a W allele and another allele for a dense pigment or piebald spotting.
  • There is a gene for dense pigment. A black, brown, or orange cat will have a DD or Dd allele combination.
  • A cat with gray, cream or light brown fur will have a dd allele combination.
  • Piebald cats with the SS and Ss allele combination have some white hair, while piebald cats with no white hair have the ss genotype.

Genetic Disorders

Genetic Disorder

You can combine the Punnett square with topics like genetic disorders. Studying how sickle cell anemia is inherited could be an interesting project for AP level students.

You can have students create a worksheet to determine the probability of a child inheriting sickle cell anemia based on the parent’s allele combinations.

Have Students Create A Species

Have students design a species from scratch to test their understanding of genetic rules. Ask them to make a list of dominant and recessive traits. Determine how many traits students will have to work with depending on how much time you want them to spend on this project.

Creating a species and determining how common some genetic traits are going to be is a great way to make sure students have a solid understanding of the Punnett square. You can have students create illustrations for the different genetic combinations.

You can go further and ask students to create genetic traits for an imaginary species, determine which traits are dominant and recessive, and ask them to create problems that other students will have to solve.


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