Introduction to the Microscope Lab
"Micro" refers to tiny, "scope" refers to view or look at. Microscopes are tools used to enlarge images of small objects so as they can be studied. The compound light microscope is an instrument containing two lenses, which magnifies, and a variety of knobs to resolve (focus) the picture. Because it uses more than one lens, it is sometimes called the compound microscope in addition to being referred to as being a light microscope. In this lab, we will learn about the proper use and handling of the microscope.
Objectives - Students will be able to:
The process known as wet-mount can be used to prepare a specimen on a slide which can be viewed with a compound light microscopes to produce an enlarged image.
I. Microscope Handling
|Give the function(s) for the numbered parts of the compound microscope.|
Part II. Preparing a wet mount of the letter "e”.
Technique for Adding a Stain when making a Wet Mount
7. Looking through the
eyepiece, move the slide to the upper right area of the
stage. What direction does the image move?
Now, move it to the lower left side of the stage. What
direction does the image move?
9. Re-center the slide and change the scope to
high power. You will notice the "e" is out of focus. Do Not touch
the coarse focus knob, instead use the fine focus to resolve the
picture. Draw the image you see of the letter e (or part of
it) on high power.
Locate the diaphragm under the stage. Move it and record the changes in light intensity as you
III. Determining Total Magnification:
Eyepiece magnification ______________